The polymerase chain reaction (PCR) is a biochemical technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis, PCR is now a common and often indispensable technique use...
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http://en.wikipedia.org/wiki/Polymerase_chain_reaction
The selective amplification of DNA by repeated cycles of (a) heat denaturation of the DNA, (b) annealing of two oligonucleotide primers that flank the DNA segment to be amplified and (c) the extension of the annealed primers with the heat insensitive Tag DNA polymerase.
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http://ppathw3.cals.cornell.edu/glossary/Defs_P.htm
a technique used to make numerous copies of a specific segment of DNA quickly and accurately. The polymerase chain reaction enables investigators to ... [3 related articles]
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http://www.britannica.com/eb/a-z/p/90
A laboratory method used to make many copies of a specific DNA sequence. Also called PCR.
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http://www.cancer.gov/dictionary?expand=P
(PCR) A fast, inexpensive technique for making an unlimited number of copies of any piece of DNA. Sometimes called 'molecular photocopying,' PCR has had an immense impact on biology and medicine, especially genetic research.
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<molecular biology, technique> The first practical system for in vitro amplification of DNA and as such one of the most important recent developments in molecular biology. ... Two synthetic oligonucleotide primers, which are complementary to two regions of the target DNA (one for each strand) to be amplified, are added to the target DNA (that...
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The first practical system for in vitro amplification of DNA, and as such one of the most important recent developments in molecular biology. Two synthetic oligonucleotide primers, which are complementary to two regions of the target DNA (one for each strand) to be amplified, are added to the target DNA (that need not be pure), in the presence of excess deoxynucleotides and Taq polymerase> Taqpolymerase, a heat-stable DNA polymerase. In a series (typically 30) of temperature cycles, the target DNA is repeatedly denatured (around 90°C), annealed to the primers (typically at 50-60°C) and a daughter strand extended from the primers (72°C). As the daughter strands themselves act as templates for subsequent cycles, DNA fragments matching both primers are amplified exponentially, rather than linearly. The original DNA need thus be neither pure nor abundant, and the PCR reaction has accordingly become widely used not only in research, but in clinical diagnostics and forensic science.
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(PCR) Type: Term Definitions: 1. an enzymatic method for the repeated copying of the two strands of DNA of a particular gene sequence. It is widely used to amplify minute quantities of biologic material so as to provide adequate specimens for laboratory study.
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an in vitro molecular technique that rapidly amplifies the number of copies of specific DNA sequences to make the amplified DNA available for other analyses
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https://courses.lumenlearning.com/microbiology/chapter/glossary/
A technique to amplify a single or few copies of a piece of DNA by several orders of magnitude, generating millions or more copies of a particular DNA sequence.
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https://www.bcm.edu/departments/molecular-virology-and-microbiology/emergin
(PCR) A technique in molecular biology, in which a single or few copies of a piece of DNA are amplified (multiplied) to yield many more copies of the part (DNA sequence) of interest. This technique can make it easier to diagnose certain infections/diseases.
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https://www.cdc.gov/parasites/glossary.html
A technique to rapidly produce many copies of a fragment of DNA.
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